Unchained Labs pushes UV/Vis quantification to the next level with innovative optics, well-controlled consumables and honed analysis techniques. UV/Vis spectroscopy is one of the oldest and most well-established methods of directly quantifying proteins, DNA and RNA. Lunatic and Stunner measure these samples within 2% of the certified value (Figure 3), easily meeting both USP and Ph. Proteins absorb most at 280 nm while DNA and RNA peak at. NIST has 3 RMs for testing UV/Vis spectrophotometers: a monoclonal antibody (NISTmAb), tryptophan and uracil – all at certified concentrations in defined buffers. The amount of light a protein, DNA or RNA sample absorbs is directly proportional to the concentration. Looking for accuracy? Lunatic and Stunner quality has been proven with Reference Materials (RMs) from the National Institute for Standards and Technology (NIST). Lunatic or Stunner read 96 of these microfluidic circuits on their SBS-compatible plates in only 10 minutes. These 2 different sized microcuvettes make the wide dynamic range possible. The 0.1 mm microcuvette detects a high maximum absorbance while the larger microcuvette is more accurate for lower absorbances. High Lunatic and Stunner plates have 2 microcuvettes in each circuit, with pathlengths measuring 0.1 and 0.7 mm. Having pathlengths that are fixed enables accuracy on Lunatic and Stunner to within 2% and precision within 1%. For more info on UV/Vis spectroscopy and Lunatic’s innovative technology, check out this tech note. The microcuvettes are part of unique microfluidic circuits that prevent any cross-contamination or evaporation while delivering highly accurate absorbance spectra. Lunatic and Stunner spectrometers perform high-speed UV/Vis spectral analysis from 230–750 nm and 0.03–275 OD with just 2 μL of sample in fixed pathlength microcuvettes (Figure 2). The best spectrometers can measure at high-throughput and with low sample volumes. UV/Vis spectrophotometers perform these measurements and calculations automatically and without any additional reagents. With Beer’s law you determine sample concentration from the absorbance at 280 nm or 260 nm (called A280 or A260), the known pathlength and the sample’s extinction coefficient or concentration factor. Proteins absorb most at 280 nm while DNA and RNA peak at 260 nm. The amount of light a protein, DNA or RNA sample absorbs is directly proportional to the concentration. Ultrafiltration & Diafiltration (UF/DF).Differential Scanning Fluorimetry (DSF).Monoclonal antibodies & recombinant proteins.Plate-based organic removal and buffer exchangeĬonfigurable end-to-end workflow solution Sizing, quality and payload quantification
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |